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Quantitation of Human Chromosomal DNA in Fingerprints and Hair Roots Using the Amelogenin Locus

Pogemiller, Jill Joann
The purpose of this study was to obtain an estimate of DNA recovery from forensic samples, such as a fingerprint and hair root. DNA from each sample was extracted, amplified, and analyzed on a 310 Genetic Analyzer. The amount of fluorescence in the X and/or Y products produced from unknown samples was compared to fluorescence in products amplified from known inputs of human DNA to create a standard curve. It was found that, depending on the set threshold, a higher percentage of males were found to be heavy shedders, and a higher percentage of females were found to be intermediate shedders. It was also found that the amount of DNA recovered from a pulled hair root supports the visual comparisons of root size ratings.