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Kinetic Study of Classical Solid Phase Peptide Synthesis Reactions

Dietrich, William Martin
A method for obtaining kinetic rate data of classical solid phase peptide synthesis reactions was developed. A small volume of the reactant solution was circulated through an ultraviolet spectrophotometer for continuous monitoring. The resulting absorbance curves were analyzed to give the desired kinetic information. Standard zero, first and second order analysis methods were used for this initial study. Rate constants were determined over a range of reaction conditions for two amino acid derivatives with useful absorbance spectra. The procedure used should be applicable to as many as 9 additional amino acid derivatives commonly used in classical solid phase peptide synthesis.