Ren, JieFrench, Donald P.Dzialowski, Andrew R.Phelps, BraylenHHMI Life Science Freshman Research Scholars2019-07-222019-07-222019-04-27Phelps, B., Ren, J., French, D. P., & Dzialowski, A. R. (2019, April 27). DNA barcoding for identifying zooplankton. Paper presented at the HHMI Life Science Freshman Research Scholars Symposium, Stillwater, OK.https://hdl.handle.net/20.500.14446/321029DNA barcoding is still a relatively new field of study that is gaining popularity as it becomes more available. But there is no single protocol that works consistently and efficiently. While there are protocols that have been developed and proven to be successful, they are for specific organisms. In this article, I took the approach of focusing on a zooplankton species, Daphnia magna, to develop a protocol that worked consistently for DNA barcoding freshwater zooplankton. A series of experiments was run to optimize each step of DNA barcoding until a control protocol was developed that could be applied to other species of zooplankton. I expect the final optimized protocol to be robust and capable of being applied to different organisms.application/pdfIn the Oklahoma State University Library's institutional repository this paper is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the article falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information.Research reportzooplanktondna barcodingmco1