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Cloning, sequencing, and identification of Phage 16, an unknown salmonella or EHEC (enterohemorrhagic E. coli) bacteriophage

Tyagi, Shrea
Nambiar, Nayna
Reddig, B. J.
Litt, P.
Jaroni, D.
Blewett, E. L.
Bacteriophage are viruses that infect, replicate and kill bacteria. Salmonella and EHEC food poisoning are caused by Salmonella and E. coli bacteria. Bacteriophage can be used to prevent food poisoning by application to food products or processing machinery. Bacteriophage P16 specifically infects Salmonella and E. coli bacteria. We cloned fragments of the P16 genome, sequence the DNA and used bioinformatics to identify P16. Phage P16 is a Salmonella phage similar to Stitch. A phylogenetic tree inferring relationships of P16 and other bacteriophage was created.