The gut microbiome plays a crucial role in maintaining host health, and probiotics have been researchered for their potential to modify microbial populations in beneficial ways. This study aimed to explore the impact of Culturelle probiotics on the gut microbiome of laboratory mice within a two-week timeframe. Despite encountering challenges in the initial phase of the study, such as unsuccessful 16S extraction from feces,cecum extraction and investigation will be used from this point forward within this research. This report provides a detailed account of the methodology, results, discussion, and conclusions derived from this investigation, integrating insights from five research articles: "Probiotic Supplementation Shapes Gut Microbiota and Prevents High-Fat Diet-Induced Metabolic Disorders in Mice" (Hsieh et al., 2021), "Microbial Modulation of Host Satiety Hormones at the Basis of Weight Control: Focus on the Endocannabinoid System" (Díaz-Ruiz et al., 2020), "Probiotics and Prebiotics: Present Status and Future Perspectives on Metabolic Disorders" (Singh et al., 2019), "Effects of Probiotic Lactobacillus Plantarum 299v Consumption Associated With Exercise Training on Weight Management" (Amaral et al., 2020), and "Gut Microbiome and Depression: How Microbes Affect the Way We Think" (Ko et al., 2022).