Adapting 3D printing to create custom devices and medications has seen significant interest in biomedical and pharmaceutical sciences. Many are exploring the usage of 3D printing to develop cell-laden structures for use in drug screening and 3D cell culture development. However, the effect on cells of bioprinting and bioinks is not well understood. The movement of cells due to electrical stimulus, known as galvanotaxis, is a phenomenon observed in many cell types including neurons, and adult stem cells. Previously our group engineered a compact galvanotaxis device that is easily adaptable to in vitro cultures. Our group observed elongation of cells after printing through a 34G nozzle. The functional effects of such changes to the cell are not well understood. Some results found indicated some cells align with the electrical field and the potential of using 3D printing for developing cell-containing devices. This study looked to expand on this research by repeating the electrical stimulus procedure on coaxial printed fibers. Coaxial structure can be found in the human body in veins, arteries, and intestines. These structures are arranged concentrically around a central lumen. The purpose of this study was to understand the role that galvanotaxis plays in the potential morphological changes of elongated human astrocyte 3D bioprinted coaxial fiber.