Fish and aquatic amphibians possess the lateral line, a sensory system used to detect water displacements. Copper is known to inactivate the neuromast organs of the lateral line system. This study investigated copper-induced neuromast loss in Xenopus laevis, the African clawed frog. X. laevis were exposed to various copper concentrations at three different life stages: newly metamorphosed juveniles, Nieuwkoop-Faber stage 51-54 larvae, and stage 54-55 larvae. Neuromasts counts, stain intensity, and behavior responses were examined. Possible behavioral effects of copper exposure to stage 54-55 larvae were investigated through an assay using air puff stimuli to produce surface waves. No change in neuromast number occurred in juvenile frogs exposed to copper at concentrations up to 3 mg/L. Neuromasts in the 51-54 trial did not photograph well, indicating that the methods of this study are better for imaging tadpoles of higher developmental stages. In the stage 54-55 trial, neuromasts were counted for four body regions: whole body, partial body, head, and tail. Although neuromast number decreased in the tail and partial body methods, this was not significantly different. Intensity of neuromasts showed a stronger concentration-dependent decrease, as a significant effect of copper concentration on intensity was observed in all four body regions. The decrease in intensity but not neuromast number may indicate that although neuromasts are still functioning, they have a decreased number of viable hair cells. Anterior neuromasts may be less sensitive to copper than posterior neuromasts. There was little difference in response to the air puff stimulus between control tadpoles and tadpoles exposed to 400 µg/L of copper. Neuromasts of X. laevis appear resistant to copper, as loss of these organs was not observed at sublethal or environmentally relevant copper concentrations. Future research could compare the resistance of neuromasts in X. laevis to species that lose the lateral line upon metamorphosis.